Thymidine Kinase 1: A Universal Marker for Cancer

Thymidine kinase as a negative selectable marker in Leishmania major.

We have tested whether the thymidine kinase gene of herpes simplex virus 1 (HSV-1 tk) could function as a negative selectable marker in Leishmania major. Several drug resistance markers have been used for positive selection of transfected DNA in trypanosomatids, including neomycin phosphotransferase (NEO; refs. 1-4) and hygromycin phosphotransferase (HYG; refs. 5,6). Negative selectable markers...

Sensitive nonradiometric method for determining thymidine kinase 1 activity.

BACKGROUND Thymidine kinase 1 (TK1) is a cytoplasmic enzyme, produced only in the S-phase of proliferating cells, that has potential as a tumor marker. Specific determination of TK1 in serum is difficult, in part because of differences in the physical properties of serum TK1 compared with cytoplasmic TK1. METHODS The first step in the new assay was phosphorylation of 3'-azido-2',3'-deoxythymi...

Serum detection of thymidine kinase 1 as a means of early detection of lung cancer.

BACKGROUND Thymidine kinase 1 (TK1) is a biomarker elevated in several malignancies, including lung cancer. Up-regulation of TK1 is an early event in carcinogenesis and therefore a target for early cancer detection. We have developed a novel Enzyme Linked Immunosorbent Assay (ELISA) to detect TK1 in serum. MATERIALS AND METHODS Forty patients with pulmonary nodules and 18 healthy individuals ...

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tie-1 protein tyrosine kinase: a novel independent prognostic marker for gastric cancer.

Protein tyrosine kinases (PTKs) are a major class of proto-oncogenes that are involved in tumor progression. The purpose of this study was to establish a comprehensive PTK expression profile in gastric cancers, with the objective of identifying possible biomarkers for gastric cancer progression. We have designed degenerate primers according to the consensus catalytic motifs to amplify PTK molec...